Our Publications
With more than twenty years experience designing, running and maintaining an HDX-MS laboratory, we can provide insight into the complexities of HDX-MS work.
"Optimization of Feasibility Stage for Hydrogen/Deuterium Exchange Mass Spectrometry"
Stephen J. Coales, Yoshitomo Hamuro.
J. Am. Soc. Mass Spectrom. (2017) DOI: 10.1007/s13361-017-1860-3.
“Characterization of the N370S Mutant of Glucocerebrosidase by Hydrogen/Deuterium Exchange Mass Spectrometry”
Liangjie Tang, Stephen J. Coales, Jeffrey A. Morrow, Tim Edmunds, Yoshitomo Hamuro.
ChemBioChem, Volume 13, Issue 15, pages 2243–2250, October 15, 2012.
"Mapping of discontinuous conformational epitopes by amide hydrogen/deuterium exchange mass spectrometry and computational docking"
Deepangi Pandit, Steven J. Tuske, Stephen J Coales, Sook Yen E, Anita Ma, Jessica E. Lee, Jeffrey A. Morrow, Jennifer F. Nemeth, and Yoshitomo Hamuro.
J. Mole. Recognition. 2012 March 25(3):114-24.
"Local Conformational Stability of HIV-1 gp120 in Unliganded and CD4-Bound States as Defined by Amide Hydrogen/Deuterium Exchange"
Leopold Kong, Chih-chin Huang, Stephen J. Coales, Kathleen S. Molnar, Jeff Skinner, Yoshitomo Hamuro, and Peter D. Kwong.
J Virol. 2010 Oct; 84(19): 10311–10321.
“Structure and dynamics of NBD1 from CFTR characterized using crystallography and hydrogen-deuterium exchange mass spectrometry”
Hal A. Lewis, Chi Wang, Xun Zhao, Yoshitomo Hamuro, Kristine Conners, Margaret C. Kearins, F. Lu, J. Michael Sauder, Kathleen S. Molnar, Stephen J. Coales, Peter C. Maloney, William B. Guggino, Diana R. Wetmore, Patricia C. Weber, and John F. Hunt.
Journal of Molecular Biology 2010, 396, 406-430.
"Expansion of time window for mass spectrometric measurement of amide hydrogen/deuterium exchange reactions"
Coales SJ, E SY, Lee JF, Ma A, Morrow JA, Hamuro Y.
Rapid Commun Mass Sp. 2010;24:3584-3592.
“Structure of IL-17A in Complex with a Potent, Fully Human Neutralizing Antibody”
Gerhardt S, Abbott WM, Hargreaves D, Pauptit RA, Davies RA, Needham MR, Langham C, Barker W, Aziz A, Snow MJ, Dawson S, Welsh F, Wilkinson T, Vaugan T, Beste G, Bishop S, Popovic B, Rees G, Sleeman M, Tuske SJ, Coales SJ, Hamuro Y, Russell C..
Journal of Molecular Biology 2009 Dec 18;394(5):905-21. Epub 2009 Oct 14.
“Stability of HIV-1 gp120 in Unliganded and CD4-Bound States as Defined by Amide Hydrogen/Deuterium Exchange”
Leopold Kong, Chih-chin Huang, Stephen J. Coales, Kathleen S. Molnar, Jeff Skinner, Yoshitomo Hamuro and Peter D. Kwong.
Journal of Virology, Vol. 84, No.19.
“A common property of amyotrophic lateral sclerosis-associated variants: Destabilization of the Cu/Zn superoxide dismutase electrostatic loop”
Kathleen S. Molnar, Qi Wang, N. Murat Karabacak, Ashutosh Tiwari, Lawrence J. Hayward, Stephen J. Coales, Yoshitomo Hamuro, Jeffrey N. Agar.
Journal of Biological Chemistry 2009, 284, 30965-30973.
“Epitope Mapping by Amide Hydrogen/Deuterium Exchange Coupled with Proteolysis and Liquid Chromatography Mass Spectrometry”
Stephen J. Coales, Steven J. Tuske, Justine C. Tomasso, and Yoshitomo Hamuro.
Rapid Communications In Mass Spectrometry, 2009; 23: 1–9.
“Identification of Pharmacological Chaperones for Gaucher Disease and Characterization of their Effects on b-Glucocerebrosidase by Hydrogen/Deuterium Exchange Mass Spectrometry”
Michael B. Tropak, Gregory Kornhaber, Brigitte Rigat, Gustavo Maegawa, Justin Buttner, Jan Blanchard, Cecilia Murphy, Steven J. Tuske, Stephen J. Coales, Yoshitomo Hamuro, Eric Brown, Don Mahuran.
ChemBioChem 2008, 9, 2650-2662.
"Isofagomine induced stabilization of glucocerebrosidase"
Kornhaber GJ, Tropak MB, Maegawa G, Tuske SJ, Coales SJ, Mahuran DJ, et al.
ChemBioChem. 2008;9(16):2643-2649.
“Use of Enhanced Peptide Amide Hydrogen/Deuterium Exchange-Mass Spectrometry (DXMS) in the Examination of Protein-Protein Interactions”
Yoshitomo Hamuro, Lora L. Hamuro, Stephen J. Coales, and Virgil L. Woods Jr..
Mass Spectrometry Analysis for Protein-Protein Interactions and Dynamics. Mark Chance Ed., Wiley-VCH, 2008.
“A Simple Test to Detect Hydrogen/Deuterium Scrambling during Gas Phase Peptide Fragmentation”
Yoshitomo Hamuro, Justine C. Tomasso, and Stephen J. Coales.
Analytical Chemistry 2008, 80, 6785-6790.
“Conformation-Dependent Ligand Regulation of ATP Hydrolysis by human KSP: Activation of Basal Hydrolysis and Inhibition of Microtubule-Stimulated Hydrolysis by a Single, Small Molecule Modulator”
Lusong Luo, Jeffrey D. Carson, Kathleen S. Molnar, Steven J. Tuske, Stephen J. Coales, Yoshitomo Hamuro, Chiu-mei Sung, Valery Sudakin, Kurt R. Auger, Dashyant Dhanak, Jeffrey R. Jackson, Pearl S. Huang, Peter J. Tummino, Robert A Copeland.
Journal of the American Chemical Society 2008, 130, 7584-7591.
“Dynamic Structural Changes During Complement C3 Activation Analyzed by Hydrogen/Deuterium Exchange Mass Spectrometry”
Michael C. Schuster, Krisztián Papp, Kathleen S. Molnar, Stephen J. Coales, Yoshitomo Hamuro, Georgia Sfyroera, Hui Chen, Michael S. Winters, and John D. Lambris.
Molecular Immunology 2008, 45, 3142-3151.
“Effects of Electrospray Capillary Temperature on Amide Hydrogen Exchange”
Stephen J. Coales, Justine C. Tomasso, and Yoshitomo Hamuro.
Rapid Communications in Mass Spectrometry 2008, 22, 1367-1371.
“Specificity of Immobilized Porcine Pepsin in H/D Exchange Compatible Conditions”
Yoshitomo Hamuro, Stephen J. Coales, Kathleen S. Molnar, Steven J. Tuske, Jeffrey A. Morrow.
Rapid Communications in Mass Spectrometry 2008, 22, 1041-1046.
“Hydrogen-Deuterium Exchange Spectrometry for Investigation of Backbone Dynamics of Oxidized and Reduced Cytochrome P450cam”
Yoshitomo Hamuro, Kathleen S. Molnar, Stephen J. Coales, Bo Ou Yang, Alana Simorellis, and Thomas C. Pochapsky.
Journal of Inorganic Biochemistry 2008, 102, 364.
“Protein-Targeting Drug Discovery Guided by Hydrogen/Deuterium Exchange Mass Spectrometry (DXMS)”
Yoshitomo Hamuro, Stephen J. Coales, and Virgil L. Woods Jr..
Mass Spectrometry in Medicinal Chemistry. Klaus T. Wanner Ed., Wiley-VCH, 2007.
“The role of protein dynamics in increasing the binding affinity of an engineered protein-protein interaction established by H/D exchange mass spectrometry”
James R. Horn, Brian Kraybill, Elizabeth J. Petro, Stephen J. Coales, Jeffrey A. Morrow, Yoshitomo Hamuro and Anthony A. Kossiakoff.
Biochemistry 2006, 45, 8488-8498.
“Hydrogen/Deuterium-Exchange (H/D-Ex) of PPARg LBD in the Presence of Various Modulators”
Yoshitomo Hamuro, Stephen J. Coales, Jeffrey A. Morrow, Kathleen S. Molnar, Steven J, Tuske, Mark R. Southern, and Patrick R. Griffin.
Protein Science 2006, 15, 1-10.
“Rapid Analysis of Protein Structure and Dynamics by Hydrogen/Deuterium Exchange Mass Spectrometry”
Yoshitomo Hamuro, Stephen J. Coales, Mark R. Southern, Jennifer F. Nemeth-Cawley, David D. Stranz, and Patrick R. Griffin.
Journal of Biomolecular Technology 2003, 14, 171-182.
(Industry Publication)
"Application of hydrogen/deuterium-exchange to p38 mitogen-activated protein kinase"
Hamuro Y, Coales SJ, Morrow JA, Griffin PR, Southern MR, Weber PC.
Am Biotechnol Lab. 2007:28.
With more than twenty years experience designing, running and maintaining an HDX-MS laboratory, we can provide insight into the complexities of HDX-MS work.
"Optimization of Feasibility Stage for Hydrogen/Deuterium Exchange Mass Spectrometry"
Stephen J. Coales, Yoshitomo Hamuro.
J. Am. Soc. Mass Spectrom. (2017) DOI: 10.1007/s13361-017-1860-3.
“Characterization of the N370S Mutant of Glucocerebrosidase by Hydrogen/Deuterium Exchange Mass Spectrometry”
Liangjie Tang, Stephen J. Coales, Jeffrey A. Morrow, Tim Edmunds, Yoshitomo Hamuro.
ChemBioChem, Volume 13, Issue 15, pages 2243–2250, October 15, 2012.
"Mapping of discontinuous conformational epitopes by amide hydrogen/deuterium exchange mass spectrometry and computational docking"
Deepangi Pandit, Steven J. Tuske, Stephen J Coales, Sook Yen E, Anita Ma, Jessica E. Lee, Jeffrey A. Morrow, Jennifer F. Nemeth, and Yoshitomo Hamuro.
J. Mole. Recognition. 2012 March 25(3):114-24.
"Local Conformational Stability of HIV-1 gp120 in Unliganded and CD4-Bound States as Defined by Amide Hydrogen/Deuterium Exchange"
Leopold Kong, Chih-chin Huang, Stephen J. Coales, Kathleen S. Molnar, Jeff Skinner, Yoshitomo Hamuro, and Peter D. Kwong.
J Virol. 2010 Oct; 84(19): 10311–10321.
“Structure and dynamics of NBD1 from CFTR characterized using crystallography and hydrogen-deuterium exchange mass spectrometry”
Hal A. Lewis, Chi Wang, Xun Zhao, Yoshitomo Hamuro, Kristine Conners, Margaret C. Kearins, F. Lu, J. Michael Sauder, Kathleen S. Molnar, Stephen J. Coales, Peter C. Maloney, William B. Guggino, Diana R. Wetmore, Patricia C. Weber, and John F. Hunt.
Journal of Molecular Biology 2010, 396, 406-430.
"Expansion of time window for mass spectrometric measurement of amide hydrogen/deuterium exchange reactions"
Coales SJ, E SY, Lee JF, Ma A, Morrow JA, Hamuro Y.
Rapid Commun Mass Sp. 2010;24:3584-3592.
“Structure of IL-17A in Complex with a Potent, Fully Human Neutralizing Antibody”
Gerhardt S, Abbott WM, Hargreaves D, Pauptit RA, Davies RA, Needham MR, Langham C, Barker W, Aziz A, Snow MJ, Dawson S, Welsh F, Wilkinson T, Vaugan T, Beste G, Bishop S, Popovic B, Rees G, Sleeman M, Tuske SJ, Coales SJ, Hamuro Y, Russell C..
Journal of Molecular Biology 2009 Dec 18;394(5):905-21. Epub 2009 Oct 14.
“Stability of HIV-1 gp120 in Unliganded and CD4-Bound States as Defined by Amide Hydrogen/Deuterium Exchange”
Leopold Kong, Chih-chin Huang, Stephen J. Coales, Kathleen S. Molnar, Jeff Skinner, Yoshitomo Hamuro and Peter D. Kwong.
Journal of Virology, Vol. 84, No.19.
“A common property of amyotrophic lateral sclerosis-associated variants: Destabilization of the Cu/Zn superoxide dismutase electrostatic loop”
Kathleen S. Molnar, Qi Wang, N. Murat Karabacak, Ashutosh Tiwari, Lawrence J. Hayward, Stephen J. Coales, Yoshitomo Hamuro, Jeffrey N. Agar.
Journal of Biological Chemistry 2009, 284, 30965-30973.
“Epitope Mapping by Amide Hydrogen/Deuterium Exchange Coupled with Proteolysis and Liquid Chromatography Mass Spectrometry”
Stephen J. Coales, Steven J. Tuske, Justine C. Tomasso, and Yoshitomo Hamuro.
Rapid Communications In Mass Spectrometry, 2009; 23: 1–9.
“Identification of Pharmacological Chaperones for Gaucher Disease and Characterization of their Effects on b-Glucocerebrosidase by Hydrogen/Deuterium Exchange Mass Spectrometry”
Michael B. Tropak, Gregory Kornhaber, Brigitte Rigat, Gustavo Maegawa, Justin Buttner, Jan Blanchard, Cecilia Murphy, Steven J. Tuske, Stephen J. Coales, Yoshitomo Hamuro, Eric Brown, Don Mahuran.
ChemBioChem 2008, 9, 2650-2662.
"Isofagomine induced stabilization of glucocerebrosidase"
Kornhaber GJ, Tropak MB, Maegawa G, Tuske SJ, Coales SJ, Mahuran DJ, et al.
ChemBioChem. 2008;9(16):2643-2649.
“Use of Enhanced Peptide Amide Hydrogen/Deuterium Exchange-Mass Spectrometry (DXMS) in the Examination of Protein-Protein Interactions”
Yoshitomo Hamuro, Lora L. Hamuro, Stephen J. Coales, and Virgil L. Woods Jr..
Mass Spectrometry Analysis for Protein-Protein Interactions and Dynamics. Mark Chance Ed., Wiley-VCH, 2008.
“A Simple Test to Detect Hydrogen/Deuterium Scrambling during Gas Phase Peptide Fragmentation”
Yoshitomo Hamuro, Justine C. Tomasso, and Stephen J. Coales.
Analytical Chemistry 2008, 80, 6785-6790.
“Conformation-Dependent Ligand Regulation of ATP Hydrolysis by human KSP: Activation of Basal Hydrolysis and Inhibition of Microtubule-Stimulated Hydrolysis by a Single, Small Molecule Modulator”
Lusong Luo, Jeffrey D. Carson, Kathleen S. Molnar, Steven J. Tuske, Stephen J. Coales, Yoshitomo Hamuro, Chiu-mei Sung, Valery Sudakin, Kurt R. Auger, Dashyant Dhanak, Jeffrey R. Jackson, Pearl S. Huang, Peter J. Tummino, Robert A Copeland.
Journal of the American Chemical Society 2008, 130, 7584-7591.
“Dynamic Structural Changes During Complement C3 Activation Analyzed by Hydrogen/Deuterium Exchange Mass Spectrometry”
Michael C. Schuster, Krisztián Papp, Kathleen S. Molnar, Stephen J. Coales, Yoshitomo Hamuro, Georgia Sfyroera, Hui Chen, Michael S. Winters, and John D. Lambris.
Molecular Immunology 2008, 45, 3142-3151.
“Effects of Electrospray Capillary Temperature on Amide Hydrogen Exchange”
Stephen J. Coales, Justine C. Tomasso, and Yoshitomo Hamuro.
Rapid Communications in Mass Spectrometry 2008, 22, 1367-1371.
“Specificity of Immobilized Porcine Pepsin in H/D Exchange Compatible Conditions”
Yoshitomo Hamuro, Stephen J. Coales, Kathleen S. Molnar, Steven J. Tuske, Jeffrey A. Morrow.
Rapid Communications in Mass Spectrometry 2008, 22, 1041-1046.
“Hydrogen-Deuterium Exchange Spectrometry for Investigation of Backbone Dynamics of Oxidized and Reduced Cytochrome P450cam”
Yoshitomo Hamuro, Kathleen S. Molnar, Stephen J. Coales, Bo Ou Yang, Alana Simorellis, and Thomas C. Pochapsky.
Journal of Inorganic Biochemistry 2008, 102, 364.
“Protein-Targeting Drug Discovery Guided by Hydrogen/Deuterium Exchange Mass Spectrometry (DXMS)”
Yoshitomo Hamuro, Stephen J. Coales, and Virgil L. Woods Jr..
Mass Spectrometry in Medicinal Chemistry. Klaus T. Wanner Ed., Wiley-VCH, 2007.
“The role of protein dynamics in increasing the binding affinity of an engineered protein-protein interaction established by H/D exchange mass spectrometry”
James R. Horn, Brian Kraybill, Elizabeth J. Petro, Stephen J. Coales, Jeffrey A. Morrow, Yoshitomo Hamuro and Anthony A. Kossiakoff.
Biochemistry 2006, 45, 8488-8498.
“Hydrogen/Deuterium-Exchange (H/D-Ex) of PPARg LBD in the Presence of Various Modulators”
Yoshitomo Hamuro, Stephen J. Coales, Jeffrey A. Morrow, Kathleen S. Molnar, Steven J, Tuske, Mark R. Southern, and Patrick R. Griffin.
Protein Science 2006, 15, 1-10.
“Rapid Analysis of Protein Structure and Dynamics by Hydrogen/Deuterium Exchange Mass Spectrometry”
Yoshitomo Hamuro, Stephen J. Coales, Mark R. Southern, Jennifer F. Nemeth-Cawley, David D. Stranz, and Patrick R. Griffin.
Journal of Biomolecular Technology 2003, 14, 171-182.
(Industry Publication)
"Application of hydrogen/deuterium-exchange to p38 mitogen-activated protein kinase"
Hamuro Y, Coales SJ, Morrow JA, Griffin PR, Southern MR, Weber PC.
Am Biotechnol Lab. 2007:28.